Scientific achievements and developments
For the entire period of the laboratory's existence
-The fundamental identity of the structure of molecules of protochlorophyll pigment of angiosperms has been proved. It has been established that protochlorophyllide is the direct precursor of chlorophyllide in the chlorophyll biosynthesis chain. It was shown that in the process of ontogenesis, the precursor of photoactive protochlorophyllide is the short-wave form of protochlorophyllide;
– Using flash photolysis and nanosecond spectroscopy, the final stages of phytochrome phototransformation have been studied, and the so-called pool of “late intermediates” has been described. The features of the metabolism of phosphoinositides and cyclic nucleotides in a plant cell are shown. The nature and role of a number of intermediates in the chain of transduction of phytochrome and phytohormonal effects has been established;
– In the field of plant photoreception, an original method has been developed for the isolation of the photoreceptor protein phytochrome from plant tissue. Using a number of physicochemical approaches, conformational differences between the red and far-red forms of fluorochrome have been established;
– The dependence of the enzymatic activity of phospholipase D (FLD) on the light conditions of plant growth and the participation of the phytochrome photoreceptor, phytohormones, and several intracellular intermediates in the mechanisms of light control of the enzymatic activity of FLD in the plant cell has been established;
– It has been shown that the specificity of cellular responses to the action of cyclic guanosine monophosphate in plant cells is due to binding sites in target proteins – components of transduction chains of vital regulatory signals. CGMP-binding plant proteins are isolated and identified using proteomics;
–Proteomic analysis was used to determine the diagnostic profile of protein expression in plants under oxidative stress;
– In the field of visual reception, the structural dynamics of the visual pigment of rhodopsin and photoreceptor membranes has been studied. Regulatory reactions in photoreceptor cells were investigated during the photocontrol of Ca2+ fluxes within membrane compartments. The related nature of cGMP in the outer segment of the retinal bacillus was established, and specific centers of its binding in the photoreceptor membrane were identified. The exchange of phosphoinositides in photoreceptor membranes was studied;
– The genes of the regulatory proteins of the retina and human lymphocytes have been cloned. The complete cDNA sequence for the Csk family tyrosine kinase from human blood lymphocytes was identified. A method for the diagnosis of eye diseases based on PCR analysis of the Csk tyrosine kinase gene has been developed;
-The concept of interaction between light and hormonal signaling cascades in the plant cell and the role of Ca2+ and cyclic mononucleotides as critical elements of signaling systems has been developed;
– It has been shown that, upon soil salinization, transduction of the intracellular signal of polyamines is realized with the participation of the cGMP-dependent signaling system and is mediated by NO;
– Methods have been developed for obtaining and increasing the biomass of mesenchymal stem cells with high viability from various sources, technologies for their induced differentiation, techniques for preparing cellular biological products on biodegradable matrices, methods for cryopreservation of cells;
– Using a combination of two-dimensional gel electrophoresis and mass spectrometric analysis, it was found that nanostructures of technogenic origin lead to significant qualitative changes in the proteome of human blood lymphocytes, a test system for monitoring the biological effect of technogenic nanomaterials on the human body was proposed;
– A laboratory optical biosensor has been developed for the determination of glucose and hydrogen peroxide on the basis of quenching the luminescence of zinc oxide. Using this biosensor as an example, the possibility of successful use of zinc oxide nanostructures as an optical sensor for glucose detection has been demonstrated.
Developments and implementations
According to the laboratory’s developments, 10 patents of the Republic of Belarus were received, 25 implementations of the developments into practice, 5 instructions for the treatment of diseases using mesenchymal stem cells, approved by the Ministry of Health of the Republic of Belarus, were carried out